Light microscopy is very common in biology research with the use of the light microscope. Since the visible light of Light Microscopy is capable in detecting small objects and it has actually a wide range of features not known for many students and professors. The light microscope practically increases its cost as an instrument, along with its versatility and quality, resulting to unavailability to most academic programs. But no matter how rare Light Microscopes could be due to its price, it has been briefly said in this article that the use of such inexpensive microscopes for students can also be very helpful for children in performing school biology experiments.
The topic here on light microscopy describes the biggest challenges especially for a beginner microscopist upon viewing the smallest objects under the microscope. Getting the right magnification is needed when viewing these tiny creatures; this is usually the first concept by these young and bright mind beginner microscopists. And it’s a big challenge for these young Microscopists to view tiny living creatures under the microscope. First of all, obtaining a sufficient contrast to what is being viewed under the microscope may not be that easy, as well as to find the focal plane. To obtain a good resolution for such specimen and to recognize the subject upon seeing it are also part of these common challenges that can be actually encountered by those who view living things under the microscope.

The bacteria, even the smallest one can be observed and recognized under the microscope at a mere 100x magnification, but are invisible under the bright field microscopes. These objects are considered as the smallest creatures that have existence on the planet.
The smallest objects that are considered to be living are the bacteria. The smallest bacteria can be observed and recognized at a mere 100x magnification
This article also features descriptions on the types of microscopes, as well as on bright field microscopy and how to use it. Mounting the specimen on the stage, optimizing the light and adjusting the condenser are also provided with proper specifications when using the microscope. Such tips as focusing, locating, and centering the specimen is stressed out as well as adjusting eyepiece separation, selecting the right objective lens for proper viewing and adjusting illumination for the objective lens that are selected. The bright field microscope is specified here on when to use it.
And lastly, it talks about the proper way to take care and to handle the microscope and also pointed it out in a quick easy step by step process. It is always advised not to use any paper material in cleaning the microscope lenses, instead, have a lens cleaner for best care.

Original text here:

http://www.ruf.rice.edu/~bioslabs/methods/microscopy/microscopy.html